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培養基配方
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CCY Modified Medium
[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
CCY Modified Medium
山東拓(tuo)普生物(wu)工程有限公(gong)司 培(pei)養基配方 //ljxinglong.com
Composition per liter:
Yeast extract..................................................... 30.0g
Casamino acids .................................................20.0g
Na2HPO4 ..........................................................2.48g
KH2PO4.............................................................0.41g
MgSO4·7H2O .................................................20.0mg
MnSO4·H2O .....................................................7.5mg
Citric acid.........................................................6.4mg
FeSO4·7H2O.....................................................6.4mg
Sodium pyruvate solution ......................... 100.00mL
pH 7.3 ± 0.2 at 25°C
Sodium Pyruvate Solution:
Composition per 100.0mL:
Sodium pyruvate...............................................23.2g
Preparation of Sodium Pyruvate Solution:
Add sodium pyruvate to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, ex
cept sodium pyruvate solution, to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly.
Adjust pH to 7.3. Autoclave for 15 min at 15 psi pres
sure–121°C. Aseptically add 100.0mL of sterile sodi
um pyruvate solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Staphylococcus aureus.
