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培養基配方

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Campylobacter Selective Medium, Karmali’s (Karmali’s Campylobacter Medium)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Campylobacter Selective

Medium, Karmali’s

(Karmali’s Campylobacter Medium)

山東拓普生物工(gong)程有限公司(si) 培養(yang)基配方(fang) //ljxinglong.com

Composition per liter:

Activated charcoal ..............................................4.0g

Columbia agar base..................................... 990.0mL

Antibiotic supplement................................... 10.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Columbia Agar Base:

Composition per 990.0mL:

Peptone..............................................................25.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Preparation of Columbia Agar Base: Add

components to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Sodium pyruvate...............................................0.05g

Cycloheximide..................................................0.05g

Cefoperazone ..................................................0.016g

Hemin..............................................................0.016g

Vancomycin ......................................................0.01g

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: Prepare 990.0mL of Co

lumbia agar base. Sterilize as directed. Cool to 50°–

55°C. Add defibrinated sheep or horse blood to a final

concentration of 5–7%. Add 10.0mL of sterile antibi

otic supplement. Mix thoroughly. For enhanced

growth, medium may also be supplemented with

0.25g of Fe2SO4·H2O, 0.25g of sodium metabisulfite,

and 0.25g of sodium pyruvate. Pour into sterile Petri

dishes. Swirl while pouring to keep charcoal in sus

pension.

Use: For the selective isolation of Campylobacter

species.

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Campylobacter Selective Medium, Karmali’s (Karmali’s Campylobacter Medium)