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Campylobacter Selective Medium, Preston’s (Preston’s Campylobacter Medium)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Campylobacter Selective Medium,
Preston’s
(Preston’s Campylobacter Medium)

山東拓普生(sheng)物工(gong)程(cheng)有限公(gong)司 培養基配(pei)方(fang) //ljxinglong.com

Composition per liter:

Campylobacter agar base............................ 940.0mL
Horse blood, lysed ........................................ 50.0mL
Antibiotic supplement................................... 10.0mL
pH 7.5 ± 0.2 at 25°C
Campylobacter Agar Base:
Composition per liter:
Agar ..................................................................12.0g
Beef extract.......................................................10.0g
Peptone .............................................................10.0g
NaCl....................................................................5.0g
Preparation of Campylobacter Agar Base:
Add components to distilled/deionized water and
bring volume to 940.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cycloheximide....................................................0.1g
Rifampicin ........................................................0.01g
Trimethoprim lactate.........................................0.01g
Polymyxin B ...................................................5000U
Preparation of Antibiotic Supplement: Add
components to 10.0mL of 50:50 acetone:distilled/
deionized water. Filter sterilize.
Preparation of Medium: To 940.0mL of cooled,
sterile Campylobacter agar base, aseptically add
50.0mL of lysed horse blood and 10.0mL of sterile
antibiotic supplement. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the selective isolation of Campylobacter
species.
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