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培養基配方

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Campylobacter Selective Medium, Butzler’s (Butzler’s Campylobacter Medium)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Campylobacter Selective

Medium, Butzler’s

(Butzler’s Campylobacter Medium)

山東拓普生(sheng)物工程(cheng)有限公司培養基配方 //ljxinglong.com

Composition per liter:

Columbia agar base..................................... 940.0mL

Blood, horse or sheep ................................... 50.0mL

Antibiotic supplement................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Agar Base:

Composition per liter:

Peptone .............................................................25.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Preparation of Columbia Agar Base: Add

components to distilled/deionized water and bring

volume to 940.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cycloheximide...............................................50.0mg

Cephazolin .....................................................15.0mg

Novobiocin ......................................................5.0mg

Bacitracin.....................................................25,000U

Colistin sulfate.............................................10,000U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: To 940.0mL of cooled,

sterile Columbia agar base, aseptically add 50.0mL of

defibrinated sheep or horse blood and 10.0mL of sterile

antibiotic supplement. Mix thoroughly. The medium

may also be supplemented with 0.25g of Fe2SO4·H2O,

0.25g of sodium metabisulfite, and 0.25g of sodium

pyruvate. Pour into sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.

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Campylobacter Selective Medium, Butzler’s (Butzler’s Campylobacter Medium)