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Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium) (Blaser’s Agar) (Campy BAP Medium)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Campylobacter Selective
Medium, Blaser-Wang
(Blaser-Wang Campylobacter Medium)
(Blaser’s Agar)
(Campy BAP Medium)

山東拓普生物工程有限公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Brucella agar base....................................... 890.0mL
Sheep blood................................................. 100.0mL
Antibiotic supplement................................... 10.0mL
Brucella Agar Base:
Composition per 890.0mL:
Agar ..................................................................15.0g
Glucose .............................................................10.0g
Pancreatic digest of casein................................10.0g
NaCl....................................................................5.0g
Peptic digest of animal tissue .............................5.0g
pH 7.5 ± 0.2 at 25°C
Preparation of Brucella Agar Base: Add com
ponents to distilled/deionized water and bring vol
ume to 890.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pres
sure–121°C. Cool to 45°–50°C.
Antibiotic Supplement:
Composition per 10.0mL:
Cephalothin....................................................15.0mg
Vancomycin ...................................................10.0mg
Trimethoprim...................................................5.0mg
Amphotericin B ...............................................2.0mg
Polymyxin B ...................................................2500U
Preparation of Antibiotic Supplement: Add
components to 10.0mL of distilled/deionized water.
Filter sterilize.
Preparation of Medium: Prepare 890.0mL of
Brucella agar base. Sterilize as directed. Cool to 50°–
55°C and add 100.0mL of sheep blood or 50.0–
70.0mL of laked horse blood. Laked blood is pre
pared by freezing whole blood overnight and thaw
ing to room temperature. Aseptically add 10.0mL of
sterile antibiotic supplement. Mix thoroughly. Pour
into sterile Petri dishes.
Use: For the selective isolation of Campylobacter
species.
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