99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

<dd id='ie9le'></dd>

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Campylobacter Selective

Medium, Blaser-Wang

(Blaser-Wang Campylobacter Medium)

山東拓普生物工(gong)程(cheng)有限公司培養基配(pei)方 //ljxinglong.com

Composition per liter:

Columbia agar base..................................... 890.0mL

Sheep blood ................................................ 100.0mL

Antibiotic supplement................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Agar Base:

Composition per liter:

Special peptone.................................................25.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Preparation of Columbia Agar Base: Add

components to distilled/deionized water and bring

volume to 890.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cephalothin....................................................15.0mg

Vancomycin ...................................................10.0mg

Trimethoprim ...................................................5.0mg

Amphotericin B................................................2.0mg

Polymyxin B ..................................................2,500U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: To 890.0mL of cooled,

sterile Columbia agar base, aseptically add 100.0mL

of sheep blood or 50.0–70.0mL of laked horse blood.

Laked blood is prepared by freezing whole blood

overnight and thawing to room temperature. Asepti

cally add 10.0mL of sterile antibiotic supplement.

Mix thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.

在線客服

Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium)