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Campylobacter Medium

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Campylobacter Medium

山(shan)東(dong)拓普生物工程有限(xian)公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Sodium aspartate...............................................10.0g
MgSO4·7H2O..................................................... 1.0g
K2HPO4.............................................................0.75g
Yeast extract........................................................0.2g
CaCl2·2H2O ...................................................28.0mg
Resazurin .........................................................1.0mg
Phosphate-cysteine solution........................ 100.0mL
Trace elements solution SL-10 ....................... 1.0mL
pH 7.0 ± 0.2 at 25°C
Phosphate-Cysteine Solution:
Composition per 100.0mL:
NaH2PO4 ...........................................................0.25g
L-Cysteine·HCl .................................................0.25g
Preparation of Phosphate-Cysteine Solution:
Add components to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Auto
clave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2·4H2O .........................................................1.5g
CoCl2·6H2O .................................................190.0mg
MnCl2·4H2O ................................................100.0mg
ZnCl2..............................................................70.0mg
Na2MoO4·2H2O .............................................36.0mg
NiCl2·6H2O ....................................................24.0mg
H3BO3 ..............................................................6.0mg
CuCl2·2H2O .....................................................2.0mg
HCl (25% solution)....................................... 10.0mL
Preparation of Trace Elements Solution SL-10:
Add FeCl2·4H2O to 10.0mL of HCl solution. Mix
thoroughly. Add distilled/deionized water and bring
volume to 1.0L. Add remaining components. Mix
thoroughly.
Preparation of Medium: Add components, ex
cept phosphate-cysteine solution, to distilled/deion
ized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 100.0mL of sterile phos
phate-cysteine solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Campy
lobacter species.
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