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培養基配方

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Campylobacter Enrichment Broth (BAM M28a)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Campylobacter Enrichment Broth

(BAM M28a)

山東拓普生物工(gong)程有限(xian)公(gong)司培(pei)養基配(pei)方 //ljxinglong.com

Composition per 1016.0mL:

Peptone .............................................................10.0g

Yeast extract........................................................5.0g

Lactalbumin hydrolysate ....................................5.0g

NaCl....................................................................5.0g

α-Ketoglutamic acid...........................................1.0g

Sodium pyruvate.................................................0.5g

Na2CO3 ...............................................................0.6g

Na2S2O5 ..............................................................0.5g

Haemin..............................................................0.01g

Cefoperazone solution .................................... 4.0mL

Trimethoprim lactate solution......................... 4.0mL

Vancomycin solution ...................................... 4.0mL

Cycloheximide or amphotericin B solution.... 4.0mL

pH 7.4 ± 0.2 at 25°C

Cefoperazone Solution:

Composition per 10.0mL:

Cefoperazone ....................................................0.05g

Preparation of Cefoperazone Solution: Add

cefoperazone to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Can be stored for 5 days at 4°C, 14 days at –20°C,

and 5 months at –70°C.

Trimethoprim Solution:

Composition per 10.0mL:

Trimethoprim lactate.......................................0.066g

Preparation of Trimethoprim Solution: Add

trimethoprim lactate to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize. Alternately add 0.05g tremethoprim hydrochlo

ride to 3.0mL 0.05N HCl. Heat to 50°C. Stir until

dissolved. Add distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Can be stored for 1 year at 4°C.

Vancomycin Solution:

Composition per 10.0mL:

Vancomycin ......................................................0.05g

Preparation of Vancomycin Solution: Add

vancomycin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Can be stored for 2 months at 4°C.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide................................................0.025g

Ethanol............................................................ 2.0mL

Preparation of Cycloheximide Solution: Add

cycloheximide to 2.0mL ethanol to dissolve. Mix

thoroughly. Add distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Can be stored for 1 year at 4°C.

Amphotericin B Solution:

Composition per 10.0mL:

Amphotericin B...............................................0.005g

Preparation of Amphotericin B Solution:

Add Amphotericin B to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize. Can be stored for 1 year at –20°C.

Preparation of Medium: Add components, ex

cept antimicrobic solutions, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

25°C. Aseptically add 4.0mL of sterile cefoperazone

solution, 4.0mL of sterile trimethoprim lactate solu

tion, 4.0mL of sterile vancomycin solution, and ei

ther 4.0mL of sterile cycloheximide solution or

4.0mL of sterile amphotericin B solution. Mix thor

oughly. Aseptically distribute into sterile screw

capped tubes or bottles. Close caps tightly to reduce

O2 absorption. Use within 2 weeks.

Use: For the selective isolation and cultivation of

Campylobacter species. For the preenrichment of

Campylobacter spp. in food samples. This medium

aids resuscitation of sublethally injured cells and

overcomes the damaging effects of food processing.

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Campylobacter Enrichment Broth (BAM M28a)