99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Campylobacter Enrichment Broth

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓(tuo)普(pu)生物工程有限公司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

Yeast extract........................................................6.0g

NaCl....................................................................5.0g

Horse blood, laked ........................................ 50.0mL

FBP solution ................................................... 4.0mL

Antibiotic solution .......................................... 4.0mL

pH 7.5 ± 0.2 at 25°C

Horse Blood, Laked:

Composition per 50.0mL:

Horse blood, fresh......................................... 50.0mL

Preparation of Horse Blood, Laked: Add

blood to a sterile polypropylene bottle. Freeze over

night at −20°C. Thaw at 8°C. Refreeze at −20°C.

Thaw again at 8°C.

FBP Solution:

Composition per 100.0mL:

FeSO4 ................................................................6.25g

NaHSO3 ............................................................6.25g

Sodium pyruvate...............................................6.25g

Preparation of FBP Solution: Add components

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Sodium cefoperazone........................................0.03g

Trimethoprim lactate.....................................0.0125g

Rifampicin ........................................................0.01g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept laked horse blood, FBP solution, and antibiotic so

lution—to distilled/deionized water and bring volume

to 942.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 50.0mL of sterile

laked horse blood, 4.0mL of FBP solution, and 4.0mL

of antibiotic solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Campylo

bacter species from dairy products.

在線客服

Campylobacter Enrichment Broth