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Campylobacter Enrichment Broth (FDA Medium M29)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Campylobacter Enrichment Broth
(FDA Medium M29)

山東拓普生物工程有限公司(si) 培養基(ji)配(pei)方(fang) //ljxinglong.com

Composition per 1024.0mL:

Basal medium ............................................. 950.0mL
Horse blood, lysed ........................................ 50.0mL
Cefoperazone solution .................................... 8.0mL
FBP solution ................................................... 4.0mL
Trimethoprim lactate solution......................... 4.0mL
Vancomycin solution....................................... 4.0mL
Cycloheximide solution .................................. 4.0mL
pH 7.5 ± 0.2 at 25°C
Basal Medium:
Composition per 950.0mL:
Beef extract.......................................................10.0g
Peptone .............................................................10.0g
Yeast extract........................................................6.0g
NaCl....................................................................5.0g
Preparation of Basal Medium: Add compo
nents to distilled/deionized water and bring volume
to 950.0mL. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C.
FBP Solution:
Composition per 100.0mL:
FeSO4·7H2O......................................................6.25g
Na2S2O5 ............................................................6.25g
Sodium pyruvate...............................................6.25g
Preparation of FBP: Add components to dis
tilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Cefoperazone Solution:
Composition per 10.0mL:
Cefoperazone ..................................................0.037g
Preparation of Cefoperazone Solution: Add
cefoperazone to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Trimethoprim Lactate Solution:
Composition per 10.0mL:
Trimethoprim lactate.......................................0.031g
Preparation of Trimethoprim Lactate Solu
tion: Add trimethoprim lactate to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin ....................................................0.025g
Preparation of Vancomycin Solution: Add
vancomycin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide................................................0.025g
Preparation of Cycloheximide Solution: Add
cycloheximide to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 950.0mL of cooled
sterile basal medium, aseptically add 50.0mL of ly
sed (fresh, frozen, and thawed) horse blood, 4.0mL
of sterile FBP solution, 8.0mL of sterile cefopera
zone solution, 4.0mL of sterile trimethoprim lactate
solution, 4.0mL of sterile vancomycin solution, and
4.0mL of sterile cycloheximide solution. Mix thor-
oughly. Aseptically distribute into sterile screw
capped tubes or bottles. Close caps tightly to reduce
O2 absorption. Use within 2 weeks.
Use: For the selective isolation and cultivation of
Campylobacter species.
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