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Acetate Broth

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
Acetate Broth

山(shan)東拓(tuo)普生物(wu)工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Meat extract ......................................................50.0g
Glucose .............................................................10.0g
Peptone................................................................5.0g
Yeast extract........................................................5.0g
Sodium acetate buffer ................................. 100.0mL
Tween 80......................................................... 0.5mL
pH 5.4 ± 0.2 at 25°C
Sodium Acetate Buffer:
Composition per liter:
Sodium acetate·3H2O......................................272.2g
Preparation of Sodium Acetate Buffer: Add
sodium acetate to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 5.4
with glacial acetic acid. Filter sterilize.
Preparation of Medium: Add components, ex
cept sodium acetate buffer, to distilled/deionized wa
ter and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Adjust pH to 5.4.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 100.0mL of sterile so
dium acetate buffer. Mix thoroughly. Aseptically dis
tribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Leuconos
toc species and Pediococcus species.
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