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Acetate Differential Agar (Sodium Acetate Agar) (Simmons’ Citrate Agar, Modified)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
Acetate Differential Agar
(Sodium Acetate Agar)
(Simmons’ Citrate Agar, Modified)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
NaCl....................................................................5.0g
Sodium acetate....................................................2.0g
(NH4)H2PO4........................................................1.0g
K2HPO4...............................................................1.0g
MgSO4·7H2O ......................................................0.2g
Bromthymol Blue .............................................0.08g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
cold distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boil
ing. Distribute into tubes to produce a 1 cm butt and
30 cm slant. Autoclave for 15 min at 15 psi pressure–
121°C. Cool tubes in a slanted position.
Use: For the differentiation of Shigella species from
Escherichia coli and also for the differentiation of
nonfermenting Gram-negative bacteria. Bacteria that
can utilize acetate as the sole carbon source turn the
medium blue.
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