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Acetate Agar (BAM M3)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
Acetate Agar
(BAM M3)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
NaCl....................................................................5.0g
Sodium acetate....................................................2.0g
KH2PO4...............................................................1.0g
(NH4)2PO4 ..........................................................1.0g
MgSO4 ................................................................0.2g
Bromthymol blue..............................................0.08g
pH 6.7 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept MgSO4, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Adjust pH to 6.7. Add 0.2g MgSO4.
Mix thoroughly. Autoclave for 15 min at 15 psi pres
sure–121°C. Cool to 45°–50°C. Aseptically distrib
ute into tubes or flasks. For slants distribute aliquots
into tubes prior to autoclaving. After autoclaving al
low tubes to cool in inclined position to obtain a 5cm
slant.
Use: For the cultivation of Leuconostoc species.
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