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Acetamide Medium (BAM M2)

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
Acetamide Medium
(BAM M2)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Stock basal solution .................................... 400.0mL
Stock acetamide solution ............................ 100.0mL
pH 6.9 ± 0.2 at 25°C
Stock Basal Solution:
Composition per 400.0mL:
Agar ....................................................................0.5g
KH2PO4 solution, 0.5M ................................ 14.0mL
K2HPO4 solution, 0.5M .................................. 6.0mL
Preparation of Stock Basal Solution: Add
components, except PR-CV solution, to distilled/
deionized water and bring volume to 400.0mL. Mix
thoroughly. Gently heat and bring to boiling with ag
itation to dissolve agar. Add 1.0mL PR-CV solution.
PR-CV Solution:
Composition per 200.0mL:
Phenol Red..........................................................2.0g
Crystal Violet......................................................0.2g
Preparation of PR-CV Solution: Add compo
nents to distilled/deionized water and bring volume
to 200.0mL. Mix thoroughly. Add 5N NaOH while
stirring until components are dissolved.
Stock Acetamide Solution:
Composition per 100.0mL:
Acetamide...........................................................1.0g
Preparation of Stock Acetamide Solution:
Add acetamide to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Store over me
thylene chloride in a screw capped container. Can be
stored indefinitely at room temperature.
Preparation of Medium: Combine 400.0mL
stock basal solution and 100.0mL stock acetamide
solution. Mix thoroughly. Distribute into tubes or
flasks. Steam for 10 min at 100°C. Cool.
Use: For the differentiation of nonfermentative
Gram-negative bacteria, especially Pseudomonas
aeruginosa, e.g., in milk.
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