培養基配方

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Yeast Synthetic Minimal Medium

[所屬分類:培養基配方] [發布時間:2021-10-21] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

D-Glucose.................................................................................... 20.0g

Agar ............................................................................................ 15.0g

(NH4)2SO4 .................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

Inositol ....................................................................................... 2.0mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyridoxine·HCl .......................................................................... 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

FeCl3 .......................................................................................... 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

KI ............................................................................................... 0.1mg

CuSO4·5H2O............................................................................ 0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add agar to 900.0mL of distilled/deion

ized water. Mix thoroughly. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. In a separate flask, add remaining components to

100.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Aseptically combine the two sterile solutions. Mix thoroughly. Pour

into sterile Petri dishes.

Use: For the cultivation of a wide variety of heterotrophic microorgan

isms.

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