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Yeast Synthetic Medium with 5′-dTMP

[所屬分類:培養基配方] [發布時間:2021-10-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Synthetic Medium with 5′-dTMP

山東拓(tuo)普(pu)生物(wu)工程有限公司 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Glucose ....................................................................................... 20.0g
Agar ............................................................................................ 15.0g
Yeast nitrogen base without amino acids...................................... 6.7g
Casamino acids without vitamins ................................................. 2.0g
2′-Deoxythymidine-5′-monophosphate .................................... 7.2mg
Preparation of Medium: Add glucose, yeast nitrogen base without
amino acids, casamino acids without vitamins, and 2′-deoxythymi
dine-5′-monophosphate to distilled/deionized water and bring volume
to 200.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/de
ionized water and bring volume to 800.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically combine the two sterile solutions.
Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute
into sterile tubes.
Use: For the cultivation and maintenance of Saccharomyces cerevisiae.
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