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Yeast Synthetic Minimal Medium

[所屬分類:培養基配方] [發布時間:2021-10-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Synthetic Minimal Medium

山(shan)東拓普(pu)生物(wu)工程有限公司 培(pei)養基(ji)配(pei)方 //ljxinglong.com

Composition per liter:

Glucose ....................................................................................... 20.0g
Agar ............................................................................................ 15.0g
Yeast nitrogen base without amino acids...................................... 6.7g
Preparation of Medium: Add glucose and yeast nitrogen base
without amino acids to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/deion
ized water and bring volume to 900.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically combine the two sterile solutions. Mix thor
oughly. Pour into sterile Petri dishes or aseptically distribute into sterile
tubes.
Use: For the cultivation and maintenance of Pichia angusta and Sac
charomyces cerevisiae.
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