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Wilson Blair Base

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wilson Blair Base

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 30.0g
Proteose peptone No. 3............................................................... 10.0g
Glucose ....................................................................................... 10.0g
Beef extract................................................................................... 5.0g
NaCl.............................................................................................. 5.0g
Selective reagent ......................................................................70.0mL
Brilliant Green (1% solution) ....................................................4.0mL
pH 7.3 ± 0.2 at 25°C
Selective Reagent:
Composition per 320.2mL:
Solution 1...............................................................................100.0mL
Solution 2...............................................................................100.0mL
Solution 3...............................................................................100.0mL
Solution 4.................................................................................20.2mL
Preparation of Selective Reagent: Combine 100.0mL of solution
1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu
tion 4. Mix thoroughly. Gently heat to boiling until a slate-grey color
develops. Cool to 50°C.
Solution 1:
Composition per 100.0mL:
NaHSO3 ...................................................................................... 40.0g
Preparation of Solution 1: Add NaHSO3 to 100.0mL of distilled/
deionized water. Mix thoroughly.
Solution 2:
Composition per 100.0mL:
NaH2PO4 ..................................................................................... 21.0g
Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/
deionized water. Mix thoroughly.
Solution 3:
Composition per 100.0mL:
Bismuth ammonium citrate......................................................... 12.5g
Preparation of Solution 3: Add bismuth ammonium citrate to
100.0mL of distilled/deionized water. Mix thoroughly.
Solution 4:
Composition per 20.2mL:
FeSO4 .......................................................................................... 0.96g
Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/de
ionized water. Add 0.2mL of concentrated HCl. Mix thoroughly.
Preparation of Medium: Add components, except selective re
agent and Brilliant Green solution, to distilled/deionized water and
bring volume to 976.0mL. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add selective reagent and
Brilliant Green solution. Mix thoroughly. Pour into sterile Petri dishes
or leave in tubes.
Use: For the isolation and cultivation of Salmonella, especially Salmo
nella typhi.
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