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Wilson and Blair’s Medium

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wilson and Blair’s Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 165.0mL:

Nutrient agar solution ............................................................100.0mL
Solution B ................................................................................45.0mL
Solution A................................................................................20.0mL
pH 6.8 ± 0.2 at 25°C
Nutrient Agar Solution:
Composition per 100.0mL:
Agar ............................................................................................ 1.95g
Pancreatic digest of gelatin......................................................... 0.65g
Beef extract................................................................................. 0.39g
Preparation of Nutrient Agar Solution: Aseptically add compo
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Solution A:
Composition per liter:
Na2HPO4................................................................................... 100.0g
Na2SO3...................................................................................... 100.0g
Glucose ....................................................................................... 50.0g
Bismuth ammonium citrate......................................................... 30.0g
Preparation of Solution A: Aseptically add 30.0g of bismuth am
monium citrate to 250.0mL of boiling distilled/deionized water. Asep
tically add Na2SO3 to 500.0mL of boiling distilled/deionized water.
Mix the two solutions. Add the Na2HPO4 to the boiling mixture. Cool
to 45°C. In a separate flask, aseptically add glucose to 250.0mL of ster
ile distilled/deionized water. Mix thoroughly. Aseptically add the glu
cose solution to the other cooled solution.
Solution B:
Composition per 225.0mL:
Ferric citrate.................................................................................. 2.0g
Brilliant Green ............................................................................ 0.55g
Preparation of Solution B: Aseptically add 2.0g of ferric citrate to
200.0mL of sterile distilled/deionized water. Mix thoroughly. Asepti
cally add Brilliant Green to 25.0mL of sterile distilled/deionized water.
Mix thoroughly. Aseptically combine the two solutions.
Preparation of Medium: Aseptically combine 100.0mL of nutrient
agar solution, 20.0mL of solution A, and 45.0mL of solution B. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Clostridium welchii.
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