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培養基配方

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Wilson Blair HiVeg Agar with Brilliant Green and Selective Reagent

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]

Wilson Blair HiVeg Agar

with Brilliant Green and Selective Reagent

山東拓(tuo)普生物工程有(you)限公司(si) 培養基(ji)配方(fang) //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 20.0g

Plant peptone............................................................................... 10.0g

Bismuth sulfite indicator............................................................... 8.0g

Glucose ......................................................................................... 5.0g

Plant extract .................................................................................. 5.0g

Na2HPO4 ....................................................................................... 4.0g

FeSO4 ............................................................................................ 0.3g

Brilliant Green .......................................................................... 0.025g

Selective reagent ......................................................................70.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium, without selective reagent, is available as a pre

mixed powder from HiMedia.

Selective Reagent:

Composition per 320.2mL:

Solution 1...............................................................................100.0mL

Solution 2...............................................................................100.0mL

Solution 3...............................................................................100.0mL

Solution 4.................................................................................20.2mL

Preparation of Selective Reagent: Combine 100.0mL of solution

1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu

tion 4. Mix thoroughly. Gently heat to boiling until a slate-grey color

develops. Cool to 50°C.

Solution 1:

Composition per 100.0mL:

NaHSO3 ...................................................................................... 40.0g

Preparation of Solution 1: Add NaHSO3 to 100.0mL of distilled/

deionized water. Mix thoroughly.

Solution 2:

Composition per 100.0mL:

NaH2PO4..................................................................................... 21.0g

Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/

deionized water. Mix thoroughly.

Solution 3:

Composition per 100.0mL:

Bismuth ammonium citrate......................................................... 12.5g

Preparation of Solution 3: Add bismuth ammonium citrate to

100.0mL of distilled/deionized water. Mix thoroughly.

Solution 4:

Composition per 20.2mL:

FeSO4.......................................................................................... 0.96g

Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/de

ionized water. Add 0.2mL of concentrated HCl. Mix thoroughly.

Preparation of Medium: Add components, except selective re

agent and Brilliant Green solution, to distilled/deionized water and

bring volume to 976.0mL. Mix thoroughly. Gently heat and bring to

boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add selective reagent and

Brilliant Green solution. Mix thoroughly. Pour into sterile Petri dishes

or leave in tubes.

Use: For the isolation and cultivation of Salmonella, especially Salmo

nella typhi.

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Wilson Blair HiVeg Agar with Brilliant Green and Selective Reagent