培養基配方

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Urea Agar

[所屬分類:培養基配方] [發布時間:2021-12-27] [發布人:網站管理員2] [閱讀次數:] [返回]

Urea Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4·12H2O ................................................9.0g

NaCl....................................................................5.0g

KH2PO4 ..............................................................1.5g

Meat extract ........................................................1.0g

Yeast extract........................................................1.0g

MgSO4·7H2O......................................................0.2g

MnCl2·4H2O ..................................................20.0mg

CaCl2................................................................1.2mg

Glucose-urea solution ................................. 100.0mL

Glucose-Urea Solution:

Composition per 100.0mL:

Urea...................................................................10.0g

Glucose ...............................................................5.0g

Preparation of Glucose-Urea Solution: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Warm to 50°C.

Preparation of Medium: Add components, ex

cept glucose-urea solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Aseptically add 100.0mL of sterile glucose-urea so

lution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the cultivation and maintenance of Coryne

bacterium glutamicum.

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