培養基配方

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Urea Agar

[所屬分類:培養基配方] [發布時間:2021-12-27] [發布人:網站管理員2] [閱讀次數:] [返回]

Urea Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Urea...................................................................10.0g

Na2HPO4·12H2O ................................................9.0g

NaCl....................................................................5.0g

KH2PO4 ..............................................................1.5g

Meat extract ........................................................1.0g

Yeast extract........................................................1.0g

MgSO4·7H2O......................................................0.2g

MnCl2·4H2O..................................................20.0mg

CaCl2 ...............................................................1.2mg

Glucose solution ......................................... 100.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose ...............................................................5.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize. Warm to

50°C.

Preparation of Medium: Add components, ex

cept glucose solution, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptical

ly add 100.0mL of sterile glucose solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and maintenance of Coryne

bacterium glutamicum.