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Tyrosine Agar (BAM M170)

[所屬分類:培養基配方] [發布時間:2021-12-24] [發布人:網站管理員2] [閱讀次數:] [返回]
Tyrosine Agar
(BAM M170)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1100mL:

Base solution...................................................... 1.0L
Tyrosine suspension.................................... 100.0mL
pH 7.0 ± 0.2 at 25°C
Base Solution:
Composition per 1.0L:
Agar ..................................................................15.0g
Pancreatic digest of gelatin.................................5.0g
Beef extract.........................................................3.0g
Preparation of Base Solution: Add components
to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boil
ing. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 48°C.
Tyrosine Suspension:
Composition per 100.0mL:
Tyrosine ..............................................................5.0g
Preparation of Tyrosine Suspension: Add ty
rosine to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 48°C.
Preparation of Medium: Combine 1.0L sterile
base solution with 100.0mL sterile tyrosine suspen
sion. Mix thoroughly by swirling or inverting several
times. Distribute into sterile tubes or pour into sterile
Petri dishes. Cool rapidly to prevent tyrosine from
separating. For slants allow tubes to cool in an in
clined position.
Use: For the selective isolation of Brevibacillus brevis
and Bacillus cereus.
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