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TPEY Agar (Tellurite Polymyxin Egg Yolk Agar)

[所屬分類:培養基配方] [發布時間:2021-12-22] [發布人:網站管理員2] [閱讀次數:] [返回]
TPEY Agar
(Tellurite Polymyxin Egg Yolk Agar)


山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................20.0g
Agar ..................................................................15.5g
Pancreatic digest of casein................................10.0g
Yeast extract........................................................5.0g
D-Mannitol ..........................................................5.0g
LiCl .....................................................................2.0g
Egg yolk emulsion (30% solution) ............. 100.0mL
Chapman tellurite solution............................ 10.0mL
Polymyxin B solution ..................................... 0.4mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Egg Yolk Emulsion (30% Solution):
Composition per 100.0mL:
NaCl....................................................................0.6g
Egg yolk........................................................ 30.0mL
Preparation of Egg Yolk Emulsion (30% So
lution): Add NaCl and egg yolk to distilled/deion
ized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Chapman Tellurite Solution:
Composition per 100.0mL:
K2TeO3 ................................................................1.0g
Preparation of Chapman Tellurite Solution:
Add K2TeO3 to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Filter sterilize.
Polymyxin B Solution:
Composition per 100.0mL:
Polymyxin B .......................................................1.0g
Preparation of Polymyxin B Solution: Add
polymyxin B to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components—ex
cept 30% egg yolk emulsion, Chapman tellurite solu
tion, and polymyxin B solution—to distilled/
deionized water and bring volume to 890.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 100.0mL of sterile 30%
egg yolk emulsion, 10.0mL of sterile Chapman tellu
rite solution, and 0.4mL of sterile polymyxin B solu
tion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the recovery of staphylococci from foods
and other materials.
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