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Toluidine Blue DNA Agar

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Toluidine Blue DNA Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g
NaCl..................................................................10.0g
Tris(hydroxymethyl)aminomethane buffer.........6.1g
Deoxyribonucleic acid (DNA)............................0.3g
Toluidine Blue O.............................................0.083g
CaCl2, anhydrous .............................................1.1mg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept Toluidine Blue O, to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently
heat and bring to boiling. Add Toluidine Blue O. Mix
thoroughly. Medium does not have to be sterilized if
used immediately. Pour into sterile Petri dishes or
distribute into sterile tubes. Allow tubes to cool in a
slanted position.
Use: For the cultivation and differentiation of bacte
ria based on their production of deoxyribonuclease
(DNase). Bacteria that produce DNase turn the medi
um pink.
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