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Toluidine Blue DNA Agar (BAM M148)

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Toluidine Blue DNA Agar
(BAM M148)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g
NaCl..................................................................10.0g
Tris(hydroxymethyl)aminomethane buffer.........6.1g
Deoxyribonucleic acid........................................0.3g
Toluidine Blue O.............................................0.083g
CaCl2, anhydrous.............................................1.1mg
pH 9.0 ± 0.2 at 25°C
Preparation of Medium: Add tris(hydroxymeth
yl)aminomethane buffer to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Adjust
pH to 9.0. Add the remaining components, except
Toluidine Blue O. Mix thoroughly. Gently heat and
bring to boiling. Add Toluidine Blue O. Mix thor-
oughly. If used the same day, sterilization is not nec
essary. Cool to 50°C. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and differentiation of Sta
phylococcus aureus from foods.
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