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Tetrathionate Broth, USA (TT Broth, USA)

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Tetrathionate Broth, USA
(TT Broth, USA)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Na2S2O3 ............................................................30.0g

CaCO3 ...............................................................10.0g
Casein peptone....................................................2.5g
Meat peptone ......................................................2.5g
Bile salts..............................................................1.0g
Source: This medium is available as a premixed
powder from Oxoid.
Iodine-Iodide Solution:
Composition per 20.0mL:
Iodine ..................................................................6.0g
KI ........................................................................5.0g
Preparation of Iodine-Iodide Solution: Add
iodine and KI to distilled/deionized water and bring
volume to 20.0mL. Mix thoroughly.
Preparation of Medium: Add components, ex
cept iodine solution, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Gently
heat and bring to boiling. Do not autoclave. Cool to
40°C. Add 20.0mL of iodine solution. Mix thorough
ly. Distribute into tubes in 10.0mL volumes. Use me
dium the same day it is prepared.
Use: For the selective enrichment of Salmonella spe
cies from foods.
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