培養基配方

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Tetrathionate Broth, Hajna (TT Broth, Hajna)

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]

Tetrathionate Broth, Hajna

(TT Broth, Hajna)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Na2S2O3 ............................................................38.0g

CaCO3 ...............................................................25.0g

Casein/meat peptone (50/50) ............................18.0g

NaCl....................................................................5.0g

D–Mannitol .........................................................2.5g

Yeast extract........................................................2.0g

Glucose ...............................................................0.5g

Sodium deoxycholate..........................................0.5g

Brilliant Green ..................................................0.01g

Iodine solution .............................................. 40.0mL

pH 7.5–7.8 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Iodine Solution:

Composition per 40.0mL:

KI ........................................................................8.0g

Iodine ..................................................................5.0g

Preparation of Iodine Solution: Add iodine and

KI to distilled/deionized water and bring volume to

40.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept iodine solution, to distilled/deionized water and

bring volume to 960.0mL. Mix thoroughly. Gently

heat and bring to boiling. Do not autoclave. Cool to

40°C. Add 40.0mL of iodine solution. Mix thorough

ly. Distribute into tubes in 10.0mL volumes. Use me

dium the same day it is prepared.

Use: For the isolation of Salmonella species, except

Salmonella typhi, and Arizona species from food

samples.

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