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Tergitol 7 Broth

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Tergitol 7 Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Lactose..............................................................10.0g
Yeast extract........................................................3.0g
Pancreatic digest of casein..................................2.5g
Peptic digest of animal tissue .............................2.5g
Tergitol 7.............................................................0.1g
Bromthymol Blue ..........................................25.0mg
TTC solution................................................... 3.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
TTC Solution:
Composition per 100.0mL:
Triphenyl tetrazolium chloride ...........................1.0g
Preparation of TTC Solution: Add triphenyltet
razolium chloride to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to
997.0mL. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add
3.0mL of sterile TTC solution. Mix thoroughly.
Use: For the isolation and cultivation of coliforms,
Salmonella, and other enteric bacteria.
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