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Tergitol 7 Agar

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Tergitol 7 Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Lactose..............................................................10.0g

Yeast extract........................................................3.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue..............................2.5g

Tergitol 7.............................................................0.1g

Bromthymol Blue ..........................................25.0mg

TTC solution................................................... 3.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

TTC Solution:

Composition per 100.0mL:

Triphenyl tetrazolium chloride ...........................1.0g

Preparation of TTC Solution: Add triphenyltet

razolium chloride to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

997.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 3.0mL of ster

ile TTC solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and differentiation of

coliform bacteria based on lactose fermentation. Lactose

fermenting bacteria appear as yellow colonies. Lactose

nonfermenting bacteria appear as blue colonies.