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培養基配方

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Sucrose Teepol Tellurite Agar (STT Agar)

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]

Sucrose Teepol Tellurite Agar

(STT Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g

Beef extract.........................................................1.0g

Peptone................................................................1.0g

Sucrose................................................................1.0g

NaCl....................................................................0.5g

Bromthymol Blue (0.2% solution).................. 2.5mL

Tellurite solution ............................................. 2.5mL

Sodium lauryl sulfate

(Teepol, 0.1% solution) ............................ 0.2mL

pH 8.0 ± 0.2 at 25°C

Tellurite Solution:

Composition per 100.0mL:

K2TeO3 ..............................................................0.05g

Preparation of Tellurite Solution: Add the

K2TeO3 to distilled/deionized water and bring the

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Use freshly prepared solution.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Pour into sterile Petri dishes.

Use: For the selective isolation, cultivation, and dif

ferentiation of Vibrio species based on their ability to

ferment sucrose. Vibrio cholerae appears as flat yel

low colonies. Vibrio parahaemolyticus appears as

levated green-yellow mucoid colonies.