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培養基配方

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Sucrose Agar

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]

Sucrose Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Sucrose..............................................................50.0g

Agar ..................................................................20.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

CaCO3 .................................................................3.0g

Phenylethyl alcohol ............................................3.0g

MgSO4·7H2O......................................................0.5g

MnSO4·4H2O......................................................0.5g

Tween 80.............................................................0.1g

Bromcresol Green..........................................20.0mg

Cycloheximide solution................................ 10.0mL

pH 6.2 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.01g

Preparation of Cycloheximide Solution: Add

cycloheximide to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add agar to distilled/

deionized water and bring volume to 500.0mL. Mix

thoroughly. Gently heat and bring to boiling. Add re

maining components, except cycloheximide solution

and phenylethyl alcohol, and bring volume to

990.0mL with distilled/deionized water. Adjust pH to

6.2. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 10.0mL of sterile

cycloheximide solution and 3.0g of phenylethyl alco

hol. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the isolation and cultivation of Lactobacil

lus species from brewery isolates.