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Starch Agar

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Starch Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................12.0g
Soluble starch....................................................10.0g
Beef extract.........................................................3.0g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distrib
ute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes.
Use: For the cultivation and differentiation of a vari
ety of microorganisms based on amylase production.
After incubation, starch hydrolysis is determined by
the addition of Gram’s or Lugol’s iodine solution. Or
ganisms that produce amylase appear as colonies sur
rounded by a clear zone.
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