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培養基配方

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Staphylococcus/Streptococcus Selective Medium

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]

Staphylococcus/Streptococcus

Selective Medium

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1060.0mL:

Columbia blood agar base ................................. 1.0L

Horse blood, defibrinated ............................. 50.0mL

Antibiotic inhibitor ....................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Blood Agar Base:

Composition per liter:

Special peptone.................................................23.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Source: Columbia blood agar base is available as a

premixed powder from Oxoid.

Preparation of Columbia Blood Agar Base:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Antibiotic Inhibitor:

Composition per 10.0mL:

Nalidixic acid..................................................0.015g

Colistin sulfate ..................................................0.01g

Ethanol (95% solution) ................................. 10.0mL

Preparation of Antibiotic Inhibitor: Add com

ponents to 10.0mL ethanol. Mix thoroughly. Filter

sterilize.

Preparation of Medium: To 1.0L of cooled ster

ile Columbia blood agar base, aseptically add sterile

horse blood and sterile antibiotic inhibitor. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the selective isolation of Staphylococcus

aureus and streptococci from Starch Agar

Composition per liter:

Agar ..................................................................12.0g

Soluble starch....................................................10.0g

Beef extract.........................................................3.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es.

Use: For the cultivation and differentiation of a vari

ety of microorganisms based on amylase production.

After incubation, starch hydrolysis is determined by

the addition of Gram’s or Lugol’s iodine solution. Or

ganisms that produce amylase appear as colonies sur

rounded by a clear zone.

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Staphylococcus/Streptococcus Selective Medium