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培養基配方

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Shigella Broth (BAM M136)

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]

Shigella Broth

(BAM M136)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................20.0g

NaCl....................................................................5.0g

K2HPO4...............................................................2.0g

KH2PO4...............................................................2.0g

Glucose ...............................................................1.0g

Novobiocin solution...................................... 11.1mL

Tween 80......................................................... 1.5mL

pH 7.0 ± 0.2 at 25°C

Novobiocin Solution:

Composition per liter:

Novobiocin........................................................0.05g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 988.9mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile novobiocin solution. Mix thoroughly.

Aseptically distribute into sterile tubes.

Use: For the isolation and cultivation of Shigella

species from food.