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培養基配方

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Sheep Blood Agar

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]

Sheep Blood Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Tryptone............................................................14.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Peptone, neutralized............................................4.5g

Yeast extract........................................................4.5g

Sheep blood, defibrinated ............................. 70.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components, ex

cept sheep blood, to distilled/deionized water and

bring volume to 930.0mL. Mix thoroughly. Heat with

frequent agitation and boil for 1 min to completely

dissolve. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 70.0mL of

sterile, defibrinated sheep blood. Mix thoroughly and

pour into sterile Petri dishes.

Use: For the isolation, cultivation, and detection of

hemolytic activity of streptococci and other fastidi

ous microorganisms. Specifically formulated to give

maximum recovery and improved hemolytic reac

tions with sheep blood.