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培養基配方

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Selenite Cystine Broth (BAM M134)

[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]

Selenite Cystine Broth

(BAM M134)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Na2HPO4.............................................................5.5g

Polypeptone ........................................................5.0g

KH2PO4...............................................................4.5g

Lactose................................................................4.0g

Na2SeO3·5H2O....................................................4.0g

L-Cystine ...........................................................0.01g

pH 7.0 ± 0.2 at 25°C

Caution: Sodium selenite is toxic and a potential

teratogen and care must be taken to avoid inhalation

of the powdered dye, contact with the skin, or inges

tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat. Do not autoclave. Dis

tribute into sterile tubes in 10.0mL volumes. Sterilize

for 10 min at 0 psi pressure–100°C in flowing steam.

Use: For the isolation and cultivation of Salmonella

species from feces, dairy products, and other speci

mens.