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Selenite Cystine Broth (BAM M134 Medium 1)

[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Selenite Cystine Broth
(BAM M134 Medium 1)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Na2HPO4 ...........................................................10.0g
Pancreatic digest of casein..................................5.0g
Lactose................................................................4.0g
Na2SeO3·5H2O....................................................4.0g
L-Cystine ...........................................................0.01g
pH 7.0 ± 0.2 at 25°C
Caution: Sodium selenite is toxic and a potential
teratogen and care must be taken to avoid inhalation
of the powdered dye, contact with the skin, or inges
tion, especially in pregnant laboratory workers.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat. Do not autoclave. Dis
tribute into sterile tubes in 10.0mL volumes. Sterilize
for 10 min at 0 psi pressure–100°C in flowing steam.
Use: For the isolation and cultivation of Salmonella
species from feces, dairy products, and other speci
mens.
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