培養基配方

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Salmonella Shigella Agar, Modified (SS Agar, Modified)

[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]

Salmonella Shigella Agar, Modified

(SS Agar, Modified)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................12.0g

Lactose..............................................................10.0g

Sodium citrate...................................................10.0g

Na2S2O3 ..............................................................8.5g

Bile salts..............................................................5.5g

Beef extract.........................................................5.0g

Peptone ...............................................................5.0g

Ferric citrate........................................................1.0g

Neutral Red.....................................................0.025g

Brilliant Green ...............................................0.33mg

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes in 20.0mL volumes. Allow

the surface of the plates to dry before inoculation.

Use: For the selective isolation and differentiation of

pathogenic enteric bacilli, especially those belonging

to the genus Salmonella. This medium provides bet

ter growth of Shigella species. Lactose-fermenting

bacteria such as Escherichia coli or Klebsiella pneu

moniae appear as small pink or red colonies. Lactose

nonfermenting bacteria—such as Salmonella spe

cies, Proteus species, and Shigella species—appear

as colorless colonies. Production of H2S by Salmo

nella species turns the center of the colonies black.