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培養基配方
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Salmonella Shigella Agar (SS Agar)
[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Salmonella Shigella Agar
(SS Agar)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................13.5g
Lactose..............................................................10.0g
Bile salts..............................................................8.5g
Na2S2O3 ..............................................................8.5g
Sodium citrate.....................................................8.5g
Beef extract.........................................................5.0g
Pancreatic digest of casein..................................2.5g
Peptic digest of animal tissue..............................2.5g
Ferric citrate........................................................1.0g
Neutral Red.....................................................0.025g
Brilliant Green ...............................................0.33mg
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour
into sterile Petri dishes in 20.0mL volumes. Allow
the surface of the plates to dry before inoculation.
Use: For the selective isolation and differentiation of
pathogenic enteric bacilli, especially those belonging
to the genus Salmonella. This medium is not recom
mended for the primary isolation of Shigella species.
Lactose-fermenting bacteria such as Escherichia coli
or Klebsiella pneumoniae appear as small pink or red
colonies. Lactose-nonfermenting bacteria—such as
Salmonella species, Proteus species, and Shigella
species—appear as colorless colonies. Production of
H2S by Salmonella species turns the center of the col
onies black.
