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Rambach® Agar

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Rambach® Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaC....................................................................l5.0g
Agar ..................................................................15.0g
Proplylene glycol..............................................10.5g
Peptone ...............................................................8.0g
Chromogenic mix ...............................................1.5g
Sodium deoxycholate..........................................1.0g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Heat in a boiling water-batch or in a
current of steam, while shaking from time to time.
The medium is totally suspended, if no visual parti
cles stick to the glass-wall. The medium should not
be heat-treated further. Complete dissolution with
shaking in 5-min sequences is approximately 35-40
min. Do not autoclave. Do not overheat. Cool as fast
as possible to 45°–50°C while gently shaking from
time to time. Pour into sterile Petri dishes. To prevent
any precipitate or clotting of the chromogenic-mix in
the plates, place Petri dishes during pouring proce
dure on a cool (max. 25°C) surface. The plates are
opaque and pink.
Use: For the detection of enteric bacteria, including
coliforms and Salmonella spp. Sodium deoxycholate
inhibits the accompanying Gram-positive flora. This
medium enables Salmonella spp. to be differentiated
unambiguously from other bacteria. Salmonella spp.
form a characteristic red color. In order to differenti
ate coliforms from Salmonellae, the medium con
tains a chromogene indicating the presence of β-ga
lactosidase splitting, a characteristic for coliforms.
Coliform microorganisms grow as blue-green or
blue-violet colonies. Other Enterobacteriaceae and
Gram-negative bacteria, such as Proteus, Pseudomo
nas, Shigella, S. typhi, and S. parathyphi A grow as
colorless-yellow colonies.
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