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Rambach Agar

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Rambach Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Polypropylene glycol ........................................10.5g
Peptone................................................................8.0g
NaCl....................................................................5.0g
Na-deoxycholate .................................................1.0g
Chromogenic mix ...............................................1.5g
pH 7.4 ± 0.2 at 25°C
Source: Rambach Agar is available from CHROM
agar Microbiology.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat in a boiling water bath
or steam bath. Shake periodically during heating to
dissolve components. Heating for 40 min with shak
ing every 5 min should ensure complete dissolution.
Do not overheat. Cool to 45-50°C. Pour into sterile
Petri dishes.
Use: For the differentiation of non-typhi Salmonella
spp. in foodstuffs. Salmonellae form acid with propy
lene glycol and non-typhi Salmonella spp. form red col
onies. Coliform bacteria grow as blue-green or blue-vi
olet colonies. Other Enterobacteriaceae and Gram neg
ative bacteria, such as Proteus spp., Pseudomonas spp.,
Shigella spp., Salmonella typhi, and Salomonella
paratyphi A grow as colorless or yellow colonies.
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