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Pseudomonas CN Agar

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Pseudomonas CN Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of gelatin...............................16.0g
Agar ..................................................................11.0g
Pancreatic digest of casein................................10.0g
K2SO4................................................................10.0g
MgCl2·6H2O .......................................................1.4g
CN selective supplement .............................. 10.0mL
Glycerol ........................................................ 10.0mL
CN Selective Supplement:
Cetrimide ............................................................0.1g
Sodium nalidixate ............................................7.5mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add components, ex
cept CN selective supplement, to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile CN selective supplement. Mix
thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the selective isolation and cultivation of
Pseudomonas species.
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