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Pseudomonas CFC Agar

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Pseudomonas CFC Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of gelatin...............................16.0g
Agar ..................................................................11.0g
Pancreatic digest of casein................................10.0g
K2SO4 ...............................................................10.0g
MgCl2·6H2O .......................................................1.4g
CFC selective supplement ............................ 10.0mL
Glycerol ........................................................ 10.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
CFC Selective Supplement:
Composition per 10.0mL:
Cephaloridine....................................................0.05g
Fucidin ..............................................................0.01g
Cetrimide ..........................................................0.01g
Preparation of CFC Selective Supplement:
Add components to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, ex
cept CFC selective supplement, to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile CFC selective supplement.
Mix thoroughly. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the selective isolation and cultivation of
Pseudomonas species.
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