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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium D
Composition per liter:
Casein peptone..............................................................................5.0g
K 2 HPO 4 .......................................................................................3.68g
NaCl..............................................................................................3.5g
KNO 3 ............................................................................................2.0g
Heart extract..................................................................................1.5g
Yeast extract..................................................................................1.5g
KH 2 PO 4 .......................................................................................1.32g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the microbiological assay of erythromycin using Klebsiella
pneumoniae.