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Antibiotic Assay Medium C
山東拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Antibiotic Assay Medium C
Composition per liter:
Peptone .........................................................................................6.0g
K2HPO 4 .......................................................................................3.68g
NaCl..............................................................................................3.5g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
KH2PO4 .......................................................................................1.32g
Glucose.........................................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the microbiological assay of Rifampin using Escherichia
coli, Colistimethate using Escherichia coli, erythromycin, framycetin,
gentamicin, gramicidin, kanamycin, neomycyin, and vancomycin
using Staphylococcus aureus, and gramicin using Enterococcus hirae.



