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Proskauer-Beck Medium for Mycobacterium

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Proskauer-Beck Medium
for Mycobacterium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Asparagine ..........................................................5.0g
KH2PO4...............................................................5.0g
Magnesium citrate ..............................................2.5g
MgSO4·7H2O......................................................0.6g
Glycerol ........................................................ 20.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, one
at a time, to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Make sure one salt is
totally dissolved before the next one is added. Adjust
pH to 7.8 with 40% NaOH. Autoclave for 15 min at
15 psi pressure–121°C. The pH of the medium after
autoclaving should be 7.4. Filter through Whatman
#1 filter paper to remove any precipitate. Distribute
into tubes or flasks. Autoclave again for 15 min at 15
psi pressure–121°C.
Use: For the cultivation and maintenance of Myco
bacterium tuberculosis.
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