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Propionibacterium Medium

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Propionibacterium Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Yeast extract......................................................10.0g
Na2HPO4·2H2O...................................................3.0g
KH2PO4...............................................................1.0g
Lactate solution............................................. 40.0mL
pH 7.0 ± 0.2 at 25°C
Lactate Solution:
Composition per 100.0mL:
Sodium lactate ..................................................70.0g
Preparation of Lactate Solution: Add 70.0g of
sodium lactate to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, ex
cept lactate solution, to distilled/deionized water and
bring volume to 960.0mL. Mix thoroughly. Add
40.0mL of lactate solution. Mix thoroughly. Adjust
pH to 7.0. Distribute into screw-capped tubes. Auto
clave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Propionibacterium acid
ipropionici, Propionibacterium freudenreichii, Pro
pionibacterium jensenii, and Propionibacterium
thoenii.
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