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Phenylalanine Malonate Broth

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]
Phenylalanine Malonate Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sodium malonate ................................................3.0g
DL-Phenylalanine ................................................2.0g
NaCl....................................................................2.0g
(NH4)2SO4...........................................................2.0g
Yeast extract........................................................1.0g
K2HPO4...............................................................0.6g
KH2PO4...............................................................0.4g
Bromthymol Blue ...........................................0.025g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes or flasks. Auto
clave for 10 min at 10 psi pressure–115°C.
Use: For the differentiation of Gram-negative enter
ic bacilli on the basis of malonate utilization and for
mation of pyruvic acid from phenylalanine.
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