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Phenylalanine Agar (Phenylalanine Deaminase Medium) (BAM M123)

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]
Phenylalanine Agar
(Phenylalanine Deaminase Medium)
(BAM M123)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................12.0g
NaCl....................................................................5.0g
Yeast extract........................................................3.0g
L-Phenylalanine ..................................................1.0g
Na2HPO4.............................................................1.0g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Distribute into tubes or flasks. Autoclave
for 10 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes.
Use: For the differentiation of enteric Gram-nega
tive bacilli on the basis of their ability to produce
phenylpyruvic acid from phenylalanine. After appro-
priate incubation of bacteria, ferric chloride reagent
is added on the agar. Formation of a green color in 1–
5 min indicates the production of phenylpyruvic acid.
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